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bmdm supernatant by elisa  (R&D Systems)


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    R&D Systems bmdm supernatant by elisa
    (A) Experimental schematic; BMDMs were treated with TCDC (100 µM) for 1 hour, followed by LPS (10ng/mL) for 3 hours. To activate the inflammasome, cells were also treated with ATP (1mM, 30 minutes before harvesting). (B) Gene expression of the cytokines TNF, MCP1, IL-1β, IL-10 and IL-12 in BMDMs. (C) TNF and (D) IL10 concentration in medium of BMDMs was measured by <t>ELISA.</t> (E) Protein levels of IL-1β and caspase-1 were measured in whole cell lysates of BMDMs. (F) IL-1β and (G) IL-18 concentrations in medium of BMDMs were measured by ELISA. (H) TCA uptake of BMDMs, in comparison to U2OS parental and U2OS-NTCP cells. (I) Caspase 3/7 activity was assessed as a marker of apoptotic activity in BMDMs incubated with the indicated concentrations of TCDC. n=3-6 per group. Experiments were performed 3 times and representative data are shown. Data are means ± SD. Significance was assessed with Mann-Whitney U test. * p <0.05.
    Bmdm Supernatant By Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 668 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bmdm+supernatant+by+elisa/bio_rxiv__64898__2026__04__21__719641-234-8-17?v=R%26D+Systems
    Average 97 stars, based on 668 article reviews
    bmdm supernatant by elisa - by Bioz Stars, 2026-07
    97/100 stars

    Images

    1) Product Images from "Increasing plasma bile salt levels with Bulevirtide alleviates DSS-induced colitis and LPS-induced inflammation"

    Article Title: Increasing plasma bile salt levels with Bulevirtide alleviates DSS-induced colitis and LPS-induced inflammation

    Journal: bioRxiv

    doi: 10.64898/2026.04.21.719641

    (A) Experimental schematic; BMDMs were treated with TCDC (100 µM) for 1 hour, followed by LPS (10ng/mL) for 3 hours. To activate the inflammasome, cells were also treated with ATP (1mM, 30 minutes before harvesting). (B) Gene expression of the cytokines TNF, MCP1, IL-1β, IL-10 and IL-12 in BMDMs. (C) TNF and (D) IL10 concentration in medium of BMDMs was measured by ELISA. (E) Protein levels of IL-1β and caspase-1 were measured in whole cell lysates of BMDMs. (F) IL-1β and (G) IL-18 concentrations in medium of BMDMs were measured by ELISA. (H) TCA uptake of BMDMs, in comparison to U2OS parental and U2OS-NTCP cells. (I) Caspase 3/7 activity was assessed as a marker of apoptotic activity in BMDMs incubated with the indicated concentrations of TCDC. n=3-6 per group. Experiments were performed 3 times and representative data are shown. Data are means ± SD. Significance was assessed with Mann-Whitney U test. * p <0.05.
    Figure Legend Snippet: (A) Experimental schematic; BMDMs were treated with TCDC (100 µM) for 1 hour, followed by LPS (10ng/mL) for 3 hours. To activate the inflammasome, cells were also treated with ATP (1mM, 30 minutes before harvesting). (B) Gene expression of the cytokines TNF, MCP1, IL-1β, IL-10 and IL-12 in BMDMs. (C) TNF and (D) IL10 concentration in medium of BMDMs was measured by ELISA. (E) Protein levels of IL-1β and caspase-1 were measured in whole cell lysates of BMDMs. (F) IL-1β and (G) IL-18 concentrations in medium of BMDMs were measured by ELISA. (H) TCA uptake of BMDMs, in comparison to U2OS parental and U2OS-NTCP cells. (I) Caspase 3/7 activity was assessed as a marker of apoptotic activity in BMDMs incubated with the indicated concentrations of TCDC. n=3-6 per group. Experiments were performed 3 times and representative data are shown. Data are means ± SD. Significance was assessed with Mann-Whitney U test. * p <0.05.

    Techniques Used: Gene Expression, Concentration Assay, Enzyme-linked Immunosorbent Assay, Comparison, Activity Assay, Marker, Incubation, MANN-WHITNEY

    (A) Schematic experimental overview; Slco1a/1b -/- FVB mice were injected with vehicle or Bulevirtide 2.5 µg/g, followed by LPS injection at 20 µg/g. (B) Total bile salt concentration and (C) concentration of individual bile salt species measured by HPLC. Plasma concentration of the cytokines TNF (D), IL-10 (E), IL-1β (F), IL-12p70 (G), IL-6 (H) and MCP1 (I). IL-1β was measured by ELISA, whereas other cytokines were measured by cytometric bead array. n=8 per group. Data are means ± SD. Significance was assessed with Mann-Whitney U test. * p <0.05.
    Figure Legend Snippet: (A) Schematic experimental overview; Slco1a/1b -/- FVB mice were injected with vehicle or Bulevirtide 2.5 µg/g, followed by LPS injection at 20 µg/g. (B) Total bile salt concentration and (C) concentration of individual bile salt species measured by HPLC. Plasma concentration of the cytokines TNF (D), IL-10 (E), IL-1β (F), IL-12p70 (G), IL-6 (H) and MCP1 (I). IL-1β was measured by ELISA, whereas other cytokines were measured by cytometric bead array. n=8 per group. Data are means ± SD. Significance was assessed with Mann-Whitney U test. * p <0.05.

    Techniques Used: Injection, Concentration Assay, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY



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    (A) Experimental schematic; BMDMs were treated with TCDC (100 µM) for 1 hour, followed by LPS (10ng/mL) for 3 hours. To activate the inflammasome, cells were also treated with ATP (1mM, 30 minutes before harvesting). (B) Gene expression of the cytokines TNF, MCP1, IL-1β, IL-10 and IL-12 in BMDMs. (C) TNF and (D) IL10 concentration in medium of BMDMs was measured by <t>ELISA.</t> (E) Protein levels of IL-1β and caspase-1 were measured in whole cell lysates of BMDMs. (F) IL-1β and (G) IL-18 concentrations in medium of BMDMs were measured by ELISA. (H) TCA uptake of BMDMs, in comparison to U2OS parental and U2OS-NTCP cells. (I) Caspase 3/7 activity was assessed as a marker of apoptotic activity in BMDMs incubated with the indicated concentrations of TCDC. n=3-6 per group. Experiments were performed 3 times and representative data are shown. Data are means ± SD. Significance was assessed with Mann-Whitney U test. * p <0.05.
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    (A) Experimental schematic; BMDMs were treated with TCDC (100 µM) for 1 hour, followed by LPS (10ng/mL) for 3 hours. To activate the inflammasome, cells were also treated with ATP (1mM, 30 minutes before harvesting). (B) Gene expression of the cytokines TNF, MCP1, IL-1β, IL-10 and IL-12 in BMDMs. (C) TNF and (D) IL10 concentration in medium of BMDMs was measured by <t>ELISA.</t> (E) Protein levels of IL-1β and caspase-1 were measured in whole cell lysates of BMDMs. (F) IL-1β and (G) IL-18 concentrations in medium of BMDMs were measured by ELISA. (H) TCA uptake of BMDMs, in comparison to U2OS parental and U2OS-NTCP cells. (I) Caspase 3/7 activity was assessed as a marker of apoptotic activity in BMDMs incubated with the indicated concentrations of TCDC. n=3-6 per group. Experiments were performed 3 times and representative data are shown. Data are means ± SD. Significance was assessed with Mann-Whitney U test. * p <0.05.
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    Image Search Results


    (A) Experimental schematic; BMDMs were treated with TCDC (100 µM) for 1 hour, followed by LPS (10ng/mL) for 3 hours. To activate the inflammasome, cells were also treated with ATP (1mM, 30 minutes before harvesting). (B) Gene expression of the cytokines TNF, MCP1, IL-1β, IL-10 and IL-12 in BMDMs. (C) TNF and (D) IL10 concentration in medium of BMDMs was measured by ELISA. (E) Protein levels of IL-1β and caspase-1 were measured in whole cell lysates of BMDMs. (F) IL-1β and (G) IL-18 concentrations in medium of BMDMs were measured by ELISA. (H) TCA uptake of BMDMs, in comparison to U2OS parental and U2OS-NTCP cells. (I) Caspase 3/7 activity was assessed as a marker of apoptotic activity in BMDMs incubated with the indicated concentrations of TCDC. n=3-6 per group. Experiments were performed 3 times and representative data are shown. Data are means ± SD. Significance was assessed with Mann-Whitney U test. * p <0.05.

    Journal: bioRxiv

    Article Title: Increasing plasma bile salt levels with Bulevirtide alleviates DSS-induced colitis and LPS-induced inflammation

    doi: 10.64898/2026.04.21.719641

    Figure Lengend Snippet: (A) Experimental schematic; BMDMs were treated with TCDC (100 µM) for 1 hour, followed by LPS (10ng/mL) for 3 hours. To activate the inflammasome, cells were also treated with ATP (1mM, 30 minutes before harvesting). (B) Gene expression of the cytokines TNF, MCP1, IL-1β, IL-10 and IL-12 in BMDMs. (C) TNF and (D) IL10 concentration in medium of BMDMs was measured by ELISA. (E) Protein levels of IL-1β and caspase-1 were measured in whole cell lysates of BMDMs. (F) IL-1β and (G) IL-18 concentrations in medium of BMDMs were measured by ELISA. (H) TCA uptake of BMDMs, in comparison to U2OS parental and U2OS-NTCP cells. (I) Caspase 3/7 activity was assessed as a marker of apoptotic activity in BMDMs incubated with the indicated concentrations of TCDC. n=3-6 per group. Experiments were performed 3 times and representative data are shown. Data are means ± SD. Significance was assessed with Mann-Whitney U test. * p <0.05.

    Article Snippet: IL-10, IL-1β and TNF concentrations were measured in BMDM supernatant by ELISA (cat. No. DY401, DY417, DY410, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instruction.

    Techniques: Gene Expression, Concentration Assay, Enzyme-linked Immunosorbent Assay, Comparison, Activity Assay, Marker, Incubation, MANN-WHITNEY

    (A) Schematic experimental overview; Slco1a/1b -/- FVB mice were injected with vehicle or Bulevirtide 2.5 µg/g, followed by LPS injection at 20 µg/g. (B) Total bile salt concentration and (C) concentration of individual bile salt species measured by HPLC. Plasma concentration of the cytokines TNF (D), IL-10 (E), IL-1β (F), IL-12p70 (G), IL-6 (H) and MCP1 (I). IL-1β was measured by ELISA, whereas other cytokines were measured by cytometric bead array. n=8 per group. Data are means ± SD. Significance was assessed with Mann-Whitney U test. * p <0.05.

    Journal: bioRxiv

    Article Title: Increasing plasma bile salt levels with Bulevirtide alleviates DSS-induced colitis and LPS-induced inflammation

    doi: 10.64898/2026.04.21.719641

    Figure Lengend Snippet: (A) Schematic experimental overview; Slco1a/1b -/- FVB mice were injected with vehicle or Bulevirtide 2.5 µg/g, followed by LPS injection at 20 µg/g. (B) Total bile salt concentration and (C) concentration of individual bile salt species measured by HPLC. Plasma concentration of the cytokines TNF (D), IL-10 (E), IL-1β (F), IL-12p70 (G), IL-6 (H) and MCP1 (I). IL-1β was measured by ELISA, whereas other cytokines were measured by cytometric bead array. n=8 per group. Data are means ± SD. Significance was assessed with Mann-Whitney U test. * p <0.05.

    Article Snippet: IL-10, IL-1β and TNF concentrations were measured in BMDM supernatant by ELISA (cat. No. DY401, DY417, DY410, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instruction.

    Techniques: Injection, Concentration Assay, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY